#Serial dilution serial
All wells that are part of a serial dilution should have the same Dilutionseries ID.Although TRIC is very robust against such buffer mismatches, it could affect the measurements. Failure to do this, could bias the measured data with a gradient in salt, DMSO, glycerol or other additives. The buffer in tube/well 1 and subsequent tubes/wells must be the same. Always mix small volumes with a pipette. A convenient way to prepare a 3-fold serial dilution of inhibitor is as follows: Let us say that the highest concentration of inhibitor to be tested is 1000 nM (i.e., 1 M), and we wish to serial dilute from this starting point.Always use the smallest micro reaction tubes possible (e.g. The high surface to volume ratio leads to surface adsorption even for well-behaved proteins.
500 μl or more) and then transfer into the Dianthus microwell plate. 20 μl) in large micro reaction tubes (e.g. The number (concentration) of viable microbial organisms is estimated from a single dilution plate (assay) without a need for replicate plates. Do not prepare less than 20 μl of sample. A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony.For ligands that are stored in DMSO, dilution is either performed in a way that maintains a constant level of DMSO throughout the dilution series or they are diluted in 100% DMSO and then each dilution point is diluted into buffer individually. The dilution factor can be freely determined by the user and is often adapted to obtain more data points for a transition phase or to cover a broader concentration range with fewer dilution points. This way, the ligand concentration is reduced by 50% in each dilution step. Typically, a serial 1:1 dilution (dilution factor 2 in DI.Control software) is performed by transferring one volume of ligand solution to an equal volume of buffer, mixing, and repeating this step.
0 kommentar(er)
